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INTERNATIONAL JOURNAL OF CREATIVE RESEARCH THOUGHTS - IJCRT (IJCRT.ORG)

International Peer Reviewed & Refereed Journals, Open Access Journal

IJCRT Peer-Reviewed (Refereed) Journal as Per New UGC Rules.

ISSN Approved Journal No: 2320-2882 | Impact factor: 7.97 | ESTD Year: 2013

Call For Paper - Volume 14 | Issue 8 | Month- August 2026

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  Paper Title: ANativeUnifiedVectorRelationalGraphIntelligenceFrameworkForCriminalLinkAnalysisAndExplainableEvidenceBoardGeneration

  Author Name(s): Prasun Kumar

  Published Paper ID: - IJCRT2607330

  Register Paper ID - 311705

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607330 and DOI :

  Author Country : Indian Author, India, 110086 , Delhi, 110086 , | Research Area: Science and Technology

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607330
Published Paper PDF: download.php?file=IJCRT2607330
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607330.pdf

  Your Paper Publication Details:

  Title: ANATIVEUNIFIEDVECTORRELATIONALGRAPHINTELLIGENCEFRAMEWORKFORCRIMINALLINKANALYSISANDEXPLAINABLEEVIDENCEBOARDGENERATION

 DOI (Digital Object Identifier) :

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Science and Technology

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d232-d246

 Year: July 2026

 Downloads: 36

  E-ISSN Number: 2320-2882

 Abstract

Modern criminal intelligence systems are heavily constrained by data silos and the structural fragmentation of heterogeneous records. Law enforcement operations generate vast streams of unstructured text narratives (e.g., field interrogations, witness accounts, surveillance summaries) alongside complex, highly connected relational data (e.g., asset ownership trails, deep familial lineages, judicial histories, vital status records, and hidden organizational backing matrices). Relational Database Management Systems (RDBMS) suffer from exponential performance degradation when calculating deep multi-hop network joins (>3 hops), while standalone Dense Vector Retrieval or Approximate Nearest Neighbor (ANN) vector spaces fail to capture explainable topological context, treating linked entities as isolated points. This paper introduces a highly scalable, single-system hybrid framework deployed entirely within an enterprise distributed property graph architecture (Nebula Graph). By embedding high-dimensional semantic vector properties directly into graph vertices and utilizing an inline, hardware-accelerated Cosine Similarity index, our framework eliminates the data synchronization latency, consistency overhead, and operational complexity inherent in decoupled multi-database pipelines. A Native Unified Vector-Relational Graph Intelligence Framework for Criminal Link Analysis and Explainable Evidence Board Generation. Furthermore, formalize the exact mathematical derivations for a unified multi-factor ranking equation balancing text semantic vector similarity, graph connectivity metrics, geographic alignment constants, and historical crime sentencing density. Finally, provide production-grade, executable nGQL (Nebula Graph Query Language) expressions and Python execution modules that deliver query-focused, explainable visual evidence boards. The empirical design framework demonstrates substantial capability improvements over standalone architectures, satisfying the strict requirements for modern digital forensics and court-admissible chain-of-custody tracking.


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 Keywords

Criminal Intelligence, Knowledge Graphs, Nebula Graph, Cosine Similarity, Dense Vector Embeddings, Link Analysis, Forensic Evidence Board, Graph RAG

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  Paper Title: Agentic AI Framework for Autonomous ICU Patient Monitoring Using Multi-Agent Clinical Decision Support System

  Author Name(s): Meghana T R, Dr. Naseer R, Dr. Sreenivasa B R

  Published Paper ID: - IJCRT2607329

  Register Paper ID - 311697

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607329 and DOI :

  Author Country : Indian Author, India, 577004 , Davanagere, 577004 , | Research Area: Humanities All

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607329
Published Paper PDF: download.php?file=IJCRT2607329
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607329.pdf

  Your Paper Publication Details:

  Title: AGENTIC AI FRAMEWORK FOR AUTONOMOUS ICU PATIENT MONITORING USING MULTI-AGENT CLINICAL DECISION SUPPORT SYSTEM

 DOI (Digital Object Identifier) :

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Humanities All

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d225-d231

 Year: July 2026

 Downloads: 27

  E-ISSN Number: 2320-2882

 Abstract

Artificial Intelligence is Rapidly growing Technology in Healthcare sector. ICU patient supervision is a complex process that needs to be finished right away. Usually, threshold-based techniques and labour-intensive manual labour are used to achieve this, which can add to the workload of physicians and delay the identification of patients' risks. The autonomous ICU patient monitoring system based on agentic AI aims to enhance patient surveillance and risk assessment. The multi-agent system architecture that has been suggested is based on the Crew AI Framework. Monitoring, risk prediction, warning generation, and recommendation are some of these agents. These agents are all work Together to handle patient data and analyze the vital signs, predict the mortality risks and generate prioritized clinical alerts.ML models, such as XG Boost and the Random Forest classifier, trained on a dataset comprising 15,000 records were used to predict the patient's risk levels. Based on the findings, it is possible to classify the patient's condition as either normal, warning or risk.


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 Keywords

Agentic AI, Multi-Agent System, ICU Monitoring, Crew AI, XGBoost, Random Forest, Clinical Decision Support.

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  Paper Title: Home Environment and Academic Achievement among Higher Secondary Students in Tiruvallur District

  Author Name(s): Dr.B.Venkatarathanam, Mrs.P.Sandhya

  Published Paper ID: - IJCRT2607328

  Register Paper ID - 311720

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607328 and DOI :

  Author Country : Indian Author, India, 631210 , Thiruthani, 631210 , | Research Area: Medical Science All

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607328
Published Paper PDF: download.php?file=IJCRT2607328
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607328.pdf

  Your Paper Publication Details:

  Title: HOME ENVIRONMENT AND ACADEMIC ACHIEVEMENT AMONG HIGHER SECONDARY STUDENTS IN TIRUVALLUR DISTRICT

 DOI (Digital Object Identifier) :

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Medical Science All

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d217-d224

 Year: July 2026

 Downloads: 24

  E-ISSN Number: 2320-2882

 Abstract

Background: Academic achievement is one of the important indicators of students' educational success and is related to a number of personal, social and environmental factors. These comprise the home environment which is one of the most important factors in the cognitive, emotional and academic development of students, which offers learning resources, parental guidance and a conducive learning environment. Although it is recognized as one of the area of importance, the empirical research regarding the relationship between home environment and achievement of students of higher secondary education in Tiruvallur District is still very limited. Purpose: The present study set to examine the relationship between home environment and academic achievement of the higher secondary students in Tiruvallur District and to find out the difference between the home environment and academic achievement with respect to selected demographic variables. Methodology: The normative survey design was used. The number of higher secondary students selected is 240 students from higher secondary Schools using simple random sampling method in Tiruvallur District, Tamilnadu. The standardized Home Environment Scale was used to gather data and academic achievement was assessed through students' score on the half-yearly examination. Descriptive statistics, t-tests, were used for the data collected analysis. Results: The findings indicated that there was no significant difference between gender and locality in all the measures of home environment and academic achievement. But there was a significant difference with regard to the medium of instruction. The study concludes that the positive home environment contributes to promoting students' academic achievement to a considerable extent.


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 Keywords

Home Environment, Academic Achievement, Higher Secondary Students, Family Environment, Educational Psychology and Student Performance.

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  Paper Title: DRUG LIKENESS PROPERTIES OF PHYTOCONSTITUENTS OF PSIDIUM GUAJAVA (GUAVA) AND THEIR MOLECULAR DOCKING AGAINST MONKEY POX VIRUS

  Author Name(s): Dadasaheb Patil, Amit Baburao Regade, Pratiksha Ganpati Bhadwankar

  Published Paper ID: - IJCRT2607327

  Register Paper ID - 311642

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607327 and DOI : https://doi.org/10.56975/ijcrt.v14i7.311642

  Author Country : Indian Author, India, - , -, - , | Research Area: Arts1 All

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607327
Published Paper PDF: download.php?file=IJCRT2607327
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607327.pdf

  Your Paper Publication Details:

  Title: DRUG LIKENESS PROPERTIES OF PHYTOCONSTITUENTS OF PSIDIUM GUAJAVA (GUAVA) AND THEIR MOLECULAR DOCKING AGAINST MONKEY POX VIRUS

 DOI (Digital Object Identifier) : https://doi.org/10.56975/ijcrt.v14i7.311642

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Arts1 All

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d190-d216

 Year: July 2026

 Downloads: 33

  E-ISSN Number: 2320-2882

 Abstract

Monkeypox is a worrisome new epidemic. The disease is caused by the monkeypox virus and is becoming more common worldwide, with an outbreak in 2022 spreading to Europe as part of the COVID -19 pandemic. The new outbreak is associated with new, previously undetected mutations and variants. In this study, virtual screening and molecular dynamics of secondary metabolites of Psidium guajava (guava) were used. Several compounds were predicted to bind tightly to viral proteins critical for viral replication, including molecules with high potential to bind thymidylate kinase and cysteine protease proteins, whose inhibition has been shown to suppress viral replication.


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By molecular docking, the compounds kampferol, myricetin, chlorogenic acid, and quercetin were found to have considerable binding to these two receptors.

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  Paper Title: UNDERSTANDING VAYA THROUGH MODERN AGE CLASSIFICATION: AN ANATOMICAL PERSPECTIVE ON HUMAN GROWTH, DEVELOPMENT, AND AGEING

  Author Name(s): Dr. Abdul Rouf, Dr. Suryakanth V Ghule

  Published Paper ID: - IJCRT2607326

  Register Paper ID - 311688

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607326 and DOI :

  Author Country : Indian Author, India, 560009 , Bengaluru, 560009 , | Research Area: Other area not in list

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607326
Published Paper PDF: download.php?file=IJCRT2607326
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607326.pdf

  Your Paper Publication Details:

  Title: UNDERSTANDING VAYA THROUGH MODERN AGE CLASSIFICATION: AN ANATOMICAL PERSPECTIVE ON HUMAN GROWTH, DEVELOPMENT, AND AGEING

 DOI (Digital Object Identifier) :

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Other area not in list

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d179-d189

 Year: July 2026

 Downloads: 25

  E-ISSN Number: 2320-2882

 Abstract

Background: Vaya is a fundamental Ayurvedic concept describing the sequential stages of human life based on growth, maturation, and ageing. This review examines these classical descriptions in the light of contemporary anatomical and developmental science. Objective: To correlate the Ayurvedic classification of Vaya with modern age classification and interpret its anatomical significance across the human lifespan. This review analyzes the classical descriptions of Garbhavastha, Bala Vaya, Madhyama Vaya, and Vruddha Vaya and compares them with current concepts of prenatal development, childhood, adulthood, and ageing. The study demonstrates that Ayurvedic features such as Dhatu maturation, Kapha, Pitta, and Vata predominance, and progressive changes in strength and tissue integrity closely correspond with modern anatomical, physiological, and developmental processes. Despite minor variations in age limits among different Acharyas, the functional characteristics of each stage show remarkable agreement with contemporary scientific knowledge. Integrating Ayurvedic principles with modern anatomy provides a comprehensive understanding of human growth, development, maturation, and senescence, highlighting the enduring scientific relevance of the concept of Vaya.


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Keywords: Vaya, Garbhavastha, Bala Vaya, Madhyama Vaya, Vruddha Vaya Human Growth and Development

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  Paper Title: Manifestation of the Unconscious Mind in Western Painting from Sigmund Freud's Psychoanalytic Perspective: A Study of Selected Artists

  Author Name(s): Dr. Muktanand Ramrao Nawghare

  Published Paper ID: - IJCRT2607325

  Register Paper ID - 311687

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607325 and DOI :

  Author Country : Indian Author, India, 440027 , Nagpur, 440027 , | Research Area: Arts All

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607325
Published Paper PDF: download.php?file=IJCRT2607325
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607325.pdf

  Your Paper Publication Details:

  Title: MANIFESTATION OF THE UNCONSCIOUS MIND IN WESTERN PAINTING FROM SIGMUND FREUD'S PSYCHOANALYTIC PERSPECTIVE: A STUDY OF SELECTED ARTISTS

 DOI (Digital Object Identifier) :

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Arts All

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d149-d178

 Year: July 2026

 Downloads: 30

  E-ISSN Number: 2320-2882

 Abstract


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  Paper Title: "ANTIMICROBIAL RESISTANCE PATTERNS AND BIOFILM-PRODUCING ABILITY OF CLINICAL PSEUDOMONAS AERUGINOSA ISOLATES: A HOSPITAL-BASED CROSS-SECTIONAL STUDY

  Author Name(s): Mr. Anshu Shastri, Dr. Kailash Jatav

  Published Paper ID: - IJCRT2607324

  Register Paper ID - 311736

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607324 and DOI :

  Author Country : Indian Author, India, 452016 , Indore, 452016 , | Research Area: Humanities All

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607324
Published Paper PDF: download.php?file=IJCRT2607324
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607324.pdf

  Your Paper Publication Details:

  Title: "ANTIMICROBIAL RESISTANCE PATTERNS AND BIOFILM-PRODUCING ABILITY OF CLINICAL PSEUDOMONAS AERUGINOSA ISOLATES: A HOSPITAL-BASED CROSS-SECTIONAL STUDY

 DOI (Digital Object Identifier) :

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Humanities All

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d136-d148

 Year: July 2026

 Downloads: 26

  E-ISSN Number: 2320-2882

 Abstract


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 Keywords

Keywords: Pseudomonas aeruginosa, Biofilm, Antimicrobial resistance, Multidrug resistance, Hospital-acquired infection.

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  Paper Title: Floristic Diversity of the Campus of Telangana Tribal Welfare Residential Degree College (Girls), Asifabad, Kumuram Bheem Asifabad District, Telangana, India

  Author Name(s): D. Sharadha, K. ARUNA KUMARI, Dr.A.SATYA GOWRI PARVATHI

  Published Paper ID: - IJCRT2607323

  Register Paper ID - 311569

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607323 and DOI : https://doi.org/10.56975/ijcrt.v14i7.311569

  Author Country : Indian Author, India, 533103 , Rajamahendravaram, 533103 , | Research Area: Life Sciences All

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607323
Published Paper PDF: download.php?file=IJCRT2607323
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607323.pdf

  Your Paper Publication Details:

  Title: FLORISTIC DIVERSITY OF THE CAMPUS OF TELANGANA TRIBAL WELFARE RESIDENTIAL DEGREE COLLEGE (GIRLS), ASIFABAD, KUMURAM BHEEM ASIFABAD DISTRICT, TELANGANA, INDIA

 DOI (Digital Object Identifier) : https://doi.org/10.56975/ijcrt.v14i7.311569

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Life Sciences All

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d109-d135

 Year: July 2026

 Downloads: 41

  E-ISSN Number: 2320-2882

 Abstract

Floristic surveys play a vital role in documenting plant diversity and providing baseline information for biodiversity conservation, ecological studies, and environmental management. The present study was conducted at the Telangana Tribal Welfare Residential Degree College (Girls) campus, Asifabad, Kumuram Bheem Asifabad District, Telangana, India, to document the floristic diversity of the campus. Repeated field surveys were carried out during the study period covering different seasons, and all vascular plant species occurring within the campus were recorded through direct field observations. Plant specimens were identified using standard regional floras and authenticated taxonomic literature. A total of 185 plant species belonging to different families and genera were documented, representing trees, shrubs, herbs, climbers, grasses, sedges, and aquatic plants. The checklist includes several medicinally important, economically valuable, ornamental, and ecologically significant species. The study provides the first comprehensive floristic inventory of the college campus and establishes a valuable baseline database for future taxonomic, ecological, conservation, and educational studies. The documented flora will serve as an important reference for plant identification, biodiversity monitoring, and botanical education, particularly for undergraduate students, while also contributing to the regional floristic knowledge of Kumuram Bheem Asifabad District, Telangana.


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Floristic Diversity ,Campus Flora , Biodiversity ,Kumuram Bheem Asifabad, Plant Taxonomy,Conservation

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  Paper Title: Parivarik Sahyog ka Divyang Kishor Vidyarthiyon ki Shaikshik Uplabdhi par Padne Wale Prabhav ka Adhyayan

  Author Name(s): Saroj Kumari, Dr. Neelam Tiwari

  Published Paper ID: - IJCRT2607322

  Register Paper ID - 311717

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607322 and DOI :

  Author Country : Indian Author, India, - , -, - , | Research Area: Arts All

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607322
Published Paper PDF: download.php?file=IJCRT2607322
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607322.pdf

  Your Paper Publication Details:

  Title: PARIVARIK SAHYOG KA DIVYANG KISHOR VIDYARTHIYON KI SHAIKSHIK UPLABDHI PAR PADNE WALE PRABHAV KA ADHYAYAN

 DOI (Digital Object Identifier) :

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Arts All

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d103-d108

 Year: July 2026

 Downloads: 25

  E-ISSN Number: 2320-2882

 Abstract

Parivarik Sahyog ka Divyang Kishor Vidyarthiyon ki Shaikshik Uplabdhi par Padne Wale Prabhav ka Adhyayan


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Parivarik Sahyog ka Divyang Kishor Vidyarthiyon ki Shaikshik Uplabdhi par Padne Wale Prabhav ka Adhyayan

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  Paper Title: "ISOLATION AND IDENTIFICATION OF PSEUDOMONAS AERUGINOSA WITH SPECIAL REFERENCE TO DETECTION OF BIOFILM PRODUCTION AND THE ROLE OF BIOFILM-FORMING GENES IN ANTIMICROBIAL SUSCEPTIBILITY PATTERN AT A TERTIARY CARE HOSPITAL, INDORE."

  Author Name(s): Mr. Anshu Shastri, Dr. Kailash Jatav

  Published Paper ID: - IJCRT2607321

  Register Paper ID - 311734

  Publisher Journal Name: IJPUBLICATION, IJCRT

  DOI Member ID: 10.6084/m9.doi.one.IJCRT2607321 and DOI :

  Author Country : Indian Author, India, 452016 , Indore, 452016 , | Research Area: Humanities All

Published Paper URL: http://ijcrt.org/viewfull.php?&p_id=IJCRT2607321
Published Paper PDF: download.php?file=IJCRT2607321
Published Paper PDF: http://www.ijcrt.org/papers/IJCRT2607321.pdf

  Your Paper Publication Details:

  Title: "ISOLATION AND IDENTIFICATION OF PSEUDOMONAS AERUGINOSA WITH SPECIAL REFERENCE TO DETECTION OF BIOFILM PRODUCTION AND THE ROLE OF BIOFILM-FORMING GENES IN ANTIMICROBIAL SUSCEPTIBILITY PATTERN AT A TERTIARY CARE HOSPITAL, INDORE."

 DOI (Digital Object Identifier) :

 Pubished in Volume: 14  | Issue: 7  | Year: July 2026

 Publisher Name : IJCRT | www.ijcrt.org | ISSN : 2320-2882

 Subject Area: Humanities All

 Author type: Indian Author

 Pubished in Volume: 14

 Issue: 7

 Pages: d90-d102

 Year: July 2026

 Downloads: 30

  E-ISSN Number: 2320-2882

 Abstract

Background Pseudomonas aeruginosa is one of the most important opportunistic pathogens responsible for hospital-acquired infections. Its remarkable ability to produce biofilms significantly contributes to antimicrobial resistance, persistent infections, prolonged hospitalization, and increased mortality. Biofilm-associated genes such as algD, pelA, pslA, and lasB play a vital role in biofilm formation and bacterial virulence. Understanding the association between biofilm production, biofilm-forming genes, and antimicrobial resistance is essential for improving infection control strategies and therapeutic outcomes. Objectives 1. To isolate and identify Pseudomonas aeruginosa from various clinical specimens. 2. To detect biofilm production using the Tissue Culture Plate (TCP) method. 3. To identify biofilm-forming genes using Polymerase Chain Reaction (PCR). 4. To evaluate antimicrobial susceptibility patterns. 5. To determine the association between biofilm formation, biofilm genes, and multidrug resistance. Materials and Methods A hospital-based cross-sectional study was conducted over 12 months in the Department of Microbiology of a tertiary care hospital in Indore, Madhya Pradesh. A total of 180 non-duplicate clinical isolates of P. aeruginosa were recovered from urine, pus, wound swabs, respiratory secretions, blood, and other clinical specimens. Identification was performed using standard microbiological methods and biochemical tests. Antimicrobial susceptibility testing was carried out according to CLSI guidelines using the Kirby-Bauer disc diffusion method. Biofilm production was assessed by the microtiter plate assay, and PCR was used to detect algD, pelA, pslA, and lasB genes. Results Among the 180 isolates, 112 (62.2%) were biofilm producers, including 41 (22.8%) strong, 46 (25.6%) moderate, and 25 (13.9%) weak producers. The prevalence of biofilm-associated genes was algD (90.2%), pslA (84.8%), pelA (79.5%), and lasB (92.0%) among biofilm-producing isolates. Biofilm producers demonstrated significantly higher resistance to ceftazidime (74.1%), ciprofloxacin (68.8%), meropenem (57.1%), imipenem (54.5%), and gentamicin (63.4%) compared with non-biofilm producers (p<0.001). Multidrug resistance was observed in 61.6% of biofilm producers compared with 23.5% of non-biofilm producers. Conclusion Biofilm formation is highly prevalent among clinical isolates of Pseudomonas aeruginosa and is strongly associated with multidrug resistance. Detection of biofilm-associated genes provides valuable information regarding bacterial virulence and antimicrobial resistance, emphasizing the need for routine surveillance and effective antibiotic stewardship in tertiary care hospitals.


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Keywords: Pseudomonas aeruginosa, Biofilm, Antimicrobial Resistance, PCR, Biofilm Genes, Multidrug Resistance

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ISSN: 2320-2882
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ISSN: 2320-2882
Impact Factor: 7.97 and ISSN APPROVED
Journal Starting Year (ESTD) : 2013
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