Volume 11 | Issue 3 |

 Abstract

The aim of this work was a trial to prepare oyster mushroom-based patties with different enrichments. Patties are prepared from rehydrated oyster mushroom, oats and corn starch and primary natural flavours and condiments Oyster mushroom was rehydrated and patties were prepared with different levels of mushroom, (0, 25, 50 and 75%) The gross proximate analysis, of prepared oyster mushroom patties were determined using appropriate methods. Samples were sensory evaluated for attributes such as appearance, colour, aroma, flavour, texture, taste, and overall acceptability Results of the sensory evaluation indicate that sample treatment 3 (T3) had a golden brown appearance and a very much pleasant smell and very lovely compact and fine (chewy) texture. According to the proximate analysis of Sample T3 test results contains 272.44 / cal around 6.1 gm of protein, 56.6 gm of carbohydrates, 4.41 gm of total fat, sodium content is 42.8 mg 34.28%, Ash 4.22% and 2.1 gm of dietary fibre in 100 gm of patties sample which proves It is generally satisfactory in terms of nutrition value and organoleptic criteria.

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 Keywords

: nutritive attributes, oyster mushroom, mushroom patties

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Creative Commons Attribution 4.0 and The Open Definition

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The present work is aimed to develop and evaluate of Eco-friendly poly-herbal hair dye by using mixed herbal of henna, indigo, amla, jatamansi, black catechu, bhringraj, black cumin seeds, charcoal, alkanet root and Loha Bhasma with either aloe-vera gel or tea decoction or coconut shell oil as solvent (F1-F6). Conventional methods of hair dyeing involve use of chemicals that results unpleasant side effects like skin irritation, allergy, hair breakage, loss or damage of hair, allergic reaction, asthma, hair fall, skin rashes, itchy scalp, skin discoloration, erythema, skin cancer and unwanted effect on pregnancy. This herbal hair dyes contain only herbs and no harsh additives or chemicals. By using this which avoid such side effects and also very helpful in coloring the hair, act as hair growth promoter, hair nourisher, conditioner, anti-dandruff agent, anti-fungal, anti-microbial, anti- inflammatory, prevent skin irritation and allergic reaction. Formulated herbal hair dye was evaluated for their organoleptic, physico-chemical, micromeritics, dyeing effect, retention capacity and stability studies. In this attempt polyherbal hair dye paste containing natural colorant (formulation F2) has shown the better dyeing efficiency which produce darkest brown colour as compared to the other formulation and also stable at any place with no side effects.

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 Keywords

poly-herbal, hair dye, Loha Bhasma, henna, indigo.

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Streptomyces hygroscopicus produces rapamycin, a macrocyclic polyketide having immunosuppressive, antifungal, and anti-tumor activity. Rapamycin has made a substantial contribution to medicine. The wild strain's ability to produce, however, is quite limited. In this way, a computationally guided biotechnology technique was suggested to increase the production capacity of rapamycin. Based on its annotated genome and biochemical data, a metabolic model of Streptomyces hygroscopicus was built at the genome scale. Phylogenetic analysis of the 16S rRNA gene and genome sequences revealed that this strain unambiguously belonged to the genus Streptomyces Hygroscopicus, and its genomic features and functional genes were comprehensively analyzed and annotated. The core structure of rapamycin is derived from (4R, 5R)-4, 5-dihydrocyclohex-1-ene-carboxilic acid that is extended by polyketide synthase. Submerged fermentation is used by many strains of Streptomyces hygroscopicus to produce rapamycin. Due to the limited amount of medicine produced by the species, engineers confront a tremendous challenge in further developing and industrializing this essential polyketide. As a result, biotechnological manipulations are required for enhanced production of this critical antibiotic. The purpose of the current work was to describe the procedures for enhancing Streptomyces hygroscopicus species ability to synthesize rapamycin (Rap). Both UV radiation and N-methyl-N-nitro-N-nitrosoguanidine (NTG) have been employed as physical and chemical mutagens to increase rapamycin production. When rapamycin production is enhanced utilizing a sequential UV mutagenesis technique. It makes a significant difference to have access to a commercially viable amount of this important macrolide molecule. The present findings confirmed that various phylogenetically different streptomycetes strains produce rapamycin.

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 Keywords

Rapamycin, Strain improvement, Streptomyces hygroscopicus genome, Multipurpose medication, mutagenesis, mutagen

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Paclitaxel is a widely used chemotherapy drug typically Extracted from the Yew tree. Paclitaxel is a poly-oxygenated cyclic diterpenoids with a characteristic Taxane ring system. It is the most effective and widely used chemotherapeutic drug for the treatment of cancers and virus-related sarcoma. Paclitaxel has high activity as an anticancer agent and is widely used in hospitals and clinics. Paclitaxel is a naturally occurring chemical component that was first identified from the bark of yew trees but presents at low levels1. In recent years, lots of yew trees have been destroyed or seriously damaged by harvesting to extract paclitaxel. Although some fungi endophytic to yew have been found to produce paclitaxel, but the levels of production are too low to be useful for commercialization until now. To find endophytic fungi that can produce paclitaxel, we isolated and collected lot of endophytic fungal strains from Bhattu Buhla, Baijnath Tehsil in Kangra District Himachal Pradesh (India). Taxus Baccata distributing in Among these endophytic fungal strains, one strain that can produce paclitaxel was isolated and its production was authenticated with LC-MS. This discovery is very significant for paclitaxel manufacture and environmental protection of yew trees. Each year, approximately 8.1 million new cancer cases are diagnosed worldwide, including India. Around five hundred patients are treated by 1 kg of Paclitaxel, which requires 10 tons of bark equivalent to 300 trees. Too frequent extraction involves destroying the Yew tree source. Demand for Paclitaxel has outstripped supply, and scientists have turned to developing Paclitaxel in plant cells followed by industrial fermentation. Through Biotechnology and Genetic Engineering, we can be generating Paclitaxel in bacteria/Fungal cell cultures instead of plant cell cultures

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 Keywords

Taxus Baccata, Taxus Brevifolia, Aspergillus Fumigatus, Paclitaxel, HPLC, Cytochrome P-450, Plant Tissue Culture, 10-deacetylbaccatin III (10-DAB III), Endophytes.

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Creative Commons Attribution 4.0 and The Open Definition

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Child labour,Worldwide crisis,Human rights,Exploitation,United nations, International labour organization.

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Dhrupad, Teaching-Methodology, Pedagogy, Analysis, Voice-culture.

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 Abstract

Stress is not new to human beings. It has existed throughout the evolution. At work or in life everyone experiences stress. All face delays, deprivation failures, losses, obligations and pressures etc. These stressors occur as a result of individuals perception of and event. In same situation someone may interpret stress as non stressor, someone may interpret it as moderate stressor and someone may interpret it as a major stressor. The effects of stress are both positive and negative. Positive stress enables individual to perform with enhanced efficiency and effectiveness, while negative stress can result in medical and physical disorders. Research performed in this field suggests that stress can improve our performance. The primary goal of this research was to identify the impact of job stress on job performance of banking employees. By sampling 6 banks, 20 responses from each bank We've identified major factors that impact job performance of private banking employees.

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stress, positive and negative, job performance and banking employees

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Today's world is dynamic and ever-changing. It's become widely accepted that children born at the late end of the 20th century and subsequent generations can expect to have around five careers over the course of a lifetime. Online classes and specialised skill workshops are becoming easy ways for employees to stay up-to-date with the latest trends and skills necessary to thrive in today's modern workplace. Because of this, increasingly employers are valuing soft skills in potential employees. For most industries, soft skills such as adaptability and empathy are considered just as valuable in a new employee as the skills and qualifications they hold. Now more than ever, it is crucial that schools integrate new learning techniques to help prepare children for the future workplace. Rote and didactic learning styles have dominated the education system since the industrial revolution. With both these methods, the child plays a relatively passive role in the learning process. In more recent times, it has become common practice for teachers to integrate more active learning techniques in the classroom such as experiential learning. Experiential [learning] is a philosophy and methodology in which educators purposefully engage with students in direct experience and focused reflection in order to increase knowledge, develop skills, and clarify values. Experiential [learning] is a philosophy and methodology in which educators purposefully engage with students in direct experience and focused reflection in order to increase knowledge, develop skills, and clarify values. Unlike traditional classroom situations where students may compete with one another or remain uninvolved or unmotivated and where the instruction is highly structured, students in experiential learning situations cooperate and learn from one another in a more semi-structured approach. Instruction is designed to engage students in direct experiences which are tied to real-world problems and situations in which the instructor facilitates rather than directs student progress.

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Learning process, Secondary Students, reflection , School

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Creative Commons Attribution 4.0 and The Open Definition

 Abstract

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Consumer Behavior , Social Media , Consumer Choice , Purchasing Decision.

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Tacrolimus is a highly valued multipurpose medication with a wide range of biological features. It is known to reduce graft rejection in humans and has the potential to cure a variety of skin problems as well as anti-inflammatory macrolactams as an immunosuppressant. Due to the structural intricacy, chemical synthesis is difficult; consequently, microbial manufacture utilizing Streptomyces tsukubaensis has been favored. The yield can easily be boosted by several folds using a variety of genetic manipulation and mutagenesis approaches. Understanding the biosynthesis process of Tacrolimus has relied heavily on biotechnological approaches. Tacrolimus is a strong immunosuppressant and a polyketide macrolide generated by the Streptomyces species. It is commonly utilized as an anti-fibrotic drug. Streptomyces tsukubaensis was developed as a mutant strain utilizing a dual mutagenesis technique using mutagens (NTG +UV), and this strain demonstrated greater tacrolimus production. The genus Streptomyces includes high G+C Gram-positive bacteria, which present a characteristic life cycle closely connected with their ability to produce secondary metabolites. These bacteria are among the most ubiquitous soil microorganisms. After mutation these bacteria exhibit variation in their phenotype as well as in genotypic level.

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 Keywords

Tacrolimus, Strain improvement, Multipurpose medication , physical mutation, mutagen, phenotype, genotype

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Creative Commons Attribution 4.0 and The Open Definition